UMich Cryo-EM Workshop 2025

Agenda

Wednesday

CryoSPARC and Heterogeneity, Intro

11 – 12pm

3D Variability Analysis and 3D Flexible Refinement, Case Studies

1 – 6pm

· Basics of 3DVA & 3D Flex

· Compositional vs. continuous heterogeneity

· 3DVA vs. 3D Class

· Custom focus mask, mesh, latent trajectory

· CryoSPARC tools

Thursday

Review of Processing, Continue Case Studies

9 – 12 pm

· Advanced 3D Flex parameters

· Mesh prep w/ micelle

Sli.do Q & A

1 – 2:45pm

· Upcoming CryoSPARC features

· Bonus processing